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Catalog Number | orb392569 |
---|---|
Category | Proteins |
Description | Recombinant human TIMP2 protein is produced by our Mammalian expression system and the target gene encoding Cys27-Pro220 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | TIMP2 |
Entrez | 7077 |
UniProt ID | P16035 |
Protein Sequence | CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDPVDHHHH HH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | Metalloproteinase inhibitor 2; CSC-21K; Tissue inh Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
21.5 kDa | |
Human TIMP1, His Tag (orb257869) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Ala 207 (Accession # P01033-1). |
Unconjugated | |
95% | |
22.6 kDa | |
Human TIMP-2, His Tag (orb257870) is expressed from human 293 cells (HEK293). It contains AA Cys 27 - Pro 220 (Accession # NP_003246.1). |
Greater than 90% as determined by SDS-PAGE. | |
48.5 kDa | |
E.coli |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
KMP2120, Recombinant Human TIMP-2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Cys27-Pro220) of Human TIMP-2 fused with a His Tag at the C-terminal. |
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