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Catalog Number | orb257869 |
---|---|
Category | Proteins |
Description | Human TIMP1, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Ala 207 (Accession # P01033-1). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 21.5 kDa |
Target | TIMP-1 |
UniProt ID | P01033 |
Protein Sequence | AAH00866 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Cys 24 - Ala 207 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH00866 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.5 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human TIMP-1 protein
98.00% | |
22 kDa (predicted); 30 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Human TIMP-1 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Cys24-Ala207) of human TIMP-1 (Accession #P01033) fused with a 6��His Tag at the C-terminus. |
CLIA, EIA, LF | |
> 90% as determined by SDS-PAGE. | |
36 kDa | |
HEK293 |
> 98%, determined by SDS-PAGE | |
This protein contains the human TIMP1 (NP_003245.1) (Met 1-Ala 207) with a C-terminal polyhistidine tag was expressed from HEK293 Cells. |
> 97%, determined by SDS-PAGE | |
This protein contains the mature form of human TIMP1 (NP_003245.1) (Cys 24-Ala 207) was expressed. |