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Catalog Number | orb257842 |
---|---|
Category | Proteins |
Description | Human SIRP alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111). |
Target | SIRP alpha |
Conjugation | Unconjugated |
Tag | C-9×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_001035111 |
UniProt ID | P78324 |
MW | 38.5 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Glu 31 - Arg 370 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001035111 |
SDS-PAGE analysis of Human SIRP alpha protein
Line graph illustrates about the sensitivity range of Human SIRP alpha protein using different concentrations
98.00% | |
38.6 kDa (predicted); 45-55 kDa (reducing condition, due to glycosylation) |
98.00% | |
38.6 kDa (predicted); 49.9 kDa (reducing condition, due to glycosylation) |
98.00% | |
39.9 kDa (predicted). Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result. |
98.00% | |
40 kDa (predicted). Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
98.00% | |
39.8 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |