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Catalog Number | orb612120 |
---|---|
Category | Proteins |
Description | Human Siglec-9, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 348 (Accession # Q9Y336-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 37.9 kDa |
Target | Siglec-9 |
UniProt ID | Q9Y336 |
Protein Sequence | NP_055256.1 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 18 - Gly 348 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_055256.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | 25 mM MES, 150 mM NaCl, pH 5.5 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.9 kDa. The protein migrates as 53-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of SIGLEC9-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
98.00% | |
64.13 kDa (predicted); 97.5 kDa (reducing conditions) |
98.00% | |
37.48 kDa (predicted); 61.92 kDa (reducing conditions) |
98.00% | |
55-90 KDa (reducing condition) |
98.00% | |
38.9 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |