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Catalog Number | orb392418 |
---|---|
Category | Proteins |
Description | Recombinant human SCG3 protein is produced by our mammalian expression system and the target gene encoding Phe20-Leu468 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | SCG3 |
Entrez | 29106 |
UniProt ID | Q8WXD2 |
Protein Sequence | FPKPGGSQDK SLHNRELSAE RPLNEQIAEA EEDKIKKTYP PENKPGQSNY SFVDNLNLLK AITEKEKIEK ERQSIRSSPL DNKLNVEDVD STKNRKLIDD YDSTKSGLDH KFQDDPDGLH QLDGTPLTAE DIVHKIAARI YEENDRAAFD KIVSKLLNLG LITESQAHTL EDEVAEVLQK LISKEANNYE EDPNKPTSWT ENQAGKIPEK VTPMAAIQDG LAKGENDETV SNTLTLTNGL ERRTKTYSED NFEELQYFPN FYALLKSIDS EKEAKEKETL ITIMKTLIDF VKMMVKYGTI SPEEGVSYLE NLDEMIALQT KNKLEKNATD NISKLFPAPS EKSHEETDST KEEAAKMEKE YGSLKDSTKD DNSNPGGKTD EPKGKTEAYL EAIRKNIEWL KKHDKKGNKE DYDLSKMRDF INKQADAYVE KGILDKEEAE AIKRIYSSLV DHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Secretogranin-3; Secretogranin III; SgIII Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.8 kDa after removal of the signal peptide. The apparent molecular mass of SCG3-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
≥90% as determined by SDS-PAGE | |
This protein contains the human SCG3(Phe20-Leu468) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
49.3 kDa | |
E.Coli |
ELISA, WB | |
Greater than 90% by SDS-PAGE gel analyses | |
53.0 kDa | |
E.Coli |
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