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Human RBP3 protein

SKU: orb706993

Description

Recombinant Human IRBP Protein is produced by E.coli expression system and the target gene encoding Thr321-Leu630 is expressed fused with a 6His tag at the N-terminus.

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
TargetRBP3
ReactivityHuman
EndotoxinsLess than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMNHKVHHHHH HMTLRSALPG VVHCLQEVLK DYYTLVDRVP TLLQHLASMD FSTVVSEEDL VTKLNAGLQA ASEDPRLLVR AIGPTETPSW PAPDAAAEDS PGVAPELPED EAIRQALVDS VFQVSVLPGN VGYLRFDSFA DASVLGVLAP YVLRQVWEPL QDTEHLIMDL RHNPGGPSSA VPLLLSYFQG PEAGPVHLFT TYDRRTNITQ EHFSHMELPG PRYSTQRGVY LLTSHRTATA AEEFAFLMQS LGWATLVGEI TAGNLLHTRT VPLLDTPEGS LALTVPVLTF IDNHGEAWLG GGVVPDAIVL AEEALDKAQE VL

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Retinol-binding protein 3; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human RBP3 protein (orb706993)

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Available Sizes

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10 μg
$ 270.00
50 μg
$ 650.00
500 μg
$ 3,330.00
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