Human PRKG1 protein

SKU: orb392338

Description

Recombinant human PRKG1 protein is produced by our mammalian expression system and the target gene encoding Gly2-Pro686 is expressed with a 6His tag at the C-terminus.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: PRKG1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MGTLRDLQYA LQEKIEELRQ RDALIDELEL ELDQKDELIQ KLQNELDKYR SVIRPATQQA QKQSASTLQG EPRTKRQAIS AEPTAFDIQD LSHVTLPFYP KSPQSKDLIK EAILDNDFMK NLELSQIQEI VDCMYPVEYG KDSCIIKEGD VGSLVYVMED GKVEVTKEGV KLCTMGPGKV FGELAILYNC TRTATVKTLV NVKLWAIDRQ CFQTIMMRTG LIKHTEYMEF LKSVPTFQSL PEEILSKLAD VLEETHYENG EYIIRQGARG DTFFIISKGT VNVTREDSPS EDPVFLRTLG KGDWFGEKAL QGEDVRTANV IAAEAVTCLV IDRDSFKHLI GGLDDVSNKA YEDAEAKAKY EAEAAFFANL KLSDFNIIDT LGVGGFGRVE LVQLKSEESK TFAMKILKKR HIVDTRQQEH IRSEKQIMQG AHSDFIVRLY RTFKDSKYLY MLMEACLGGE LWTILRDRGS FEDSTTRFYT ACVVEAFAYL HSKGIIYRDL KPENLILDHR GYAKLVDFGF AKKIGFGKKT WTFCGTPEYV APEIILNKGH DISADYWSLG ILMYELLTGS PPFSGPDPMK TYNIILRGID MIEFPKKIAK NAANLIKKLC RDNPSERLGN LKNGVKDIQK HKWFEGFNWE GLRKGTLTPP IIPSVASPTD TSNFDSFPED NDEPPPDDNS GWDIDFVDHH HHHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 20% glycerol, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKG1B; PRKGR1A; PRKGR1B; cGMP-dependent protein kinase 1; cGK 1; cGK1; cGMP-dependent protein kinase I; cGKI

Quick Database Links

Gene Symbol

PRKG1

UniProt

Q13976

NCBI Gene ID

5592