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Human PLAU Protein, His Tag
Catalog Number: orb257779
| Catalog Number | orb257779 |
|---|---|
| Category | Proteins |
| Description | Human PLAU, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1). |
| Target | PLAU |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Sodium Acetate, 100 mM NaCl, pH 5.0 |
| Purity | 92% |
| Protein Sequence | NP_002649.1 |
| UniProt ID | P00749 |
| MW | 47.2 kDa |
| Application notes | Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.2 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Please note that the protein is not activated by protease digestion. In vitro activation is generally recommended for higher activity. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Expression Region | Ser 21 - Leu 431 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_002649.1 |
SDS-PAGE analysis of Human PLAU protein
Human PLAU Protein, His Tag [orb2697407]
> 95%, determined by SDS-PAGE
This protein contains the human PLAU (NP_002649.1) (Met 1-Leu 431) with a C-terminal polyhistidine tag was expressed from HEK293 Cells.
50 μg, 20 μgHuman PLAU Protein, His Tag [orb2697605]
> 95%, determined by SDS-PAGE
This protein contains the human PLAU (NP_002649.1) (Met 1-Leu 431) with a C-terminal polyhistidine tag was expressed from HEK293 Cells.
1 mg, 100 μgHuman PLAU Protein, His Tag [orb2701163]
> 98%, determined by SDS-PAGE
This protein contains the human UPAR isoform 1 (Q03405-1) (Met 1-Arg 303) without the pro peptide was expressed with a carboxy-terminal polyhistidine tag and expressed from HEK293 Cells.
200 μg, 100 μgHuman PLAU Protein, His Tag [orb2701241]
> 97%, determined by SDS-PAGE
This protein contains the human PLAU (NP_002649.1) (Met 1-Leu 431) with a C-terminal polyhistidine tag was expressed. The purified protein was activated by trypsin in vitro.
20 μg, 10 μgHuman PLAU Protein, His Tag [orb2701242]
> 97%, determined by SDS-PAGE
This protein contains the human PLAU (NP_002649.1) (Met 1-Leu 431) with a C-terminal polyhistidine tag was expressed from HEK293 Cells.
200 μg, 100 μg
