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Catalog Number | orb392253 |
---|---|
Category | Proteins |
Description | Recombinant human PDCD4 protein is produced by our E. coli expression system and the target gene encoding Lys212-Pro357 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | PDCD4 |
Entrez | 27250 |
UniProt ID | Q53EL6 |
Protein Sequence | MKASHREMTS KLLSDLCGTV MSTTDVEKSF DKLLKDLPEL ALDTPRAPQL VGQFIARAVG DGILCNTYID SYKGTVDCVQ ARAALDKATV LLSMSKGGKR KDSVWGSGGG QQSVNHLVKE IDMLLKEYLL SGDISEAEHC LKELEVPLEH HHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | H731; Programmed cell death protein 4; Neoplastic Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Human | |
0.05-20ng/ml | |
0.025ng/ml |
≥90% as determined by SDS-PAGE | |
This protein contains the human PDCD4(Lys212-Pro357) was fused with the C-terminal His Tag and expressed in E. coli. |
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