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Catalog Number | orb705336 |
---|---|
Category | Proteins |
Description | Recombinant Human Programmed cell death protein 1(PDCD1),partial |
Tag | C-terminal 6xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 93% as determined by SDS-PAGE. |
MW | 18.2 kDa |
UniProt ID | Q15116 |
Protein Sequence | LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ |
Protein Length | Partial |
Source | Mammalian cell |
Expression System | Expression Region: 25-167aa. Protein Length: Partial |
Biological Activity | ①Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 μg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml. |
Expression Region | 25-167aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Alternative names | (Protein PD-1) (hPD-1) (CD279) Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
42.1 kDa | |
Human PD-1, Fc Tag, low endotoxin (orb257753) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2). |
Unconjugated | |
90% | |
19.0 kDa | |
Human PD-1, Strep Tag (orb257756) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1). |
Unconjugated | |
95% | |
49.2 kDa | |
Human PD-L2, Fc Tag (HPLC verified) (orb257768) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679). |
Unconjugated | |
95% | |
16.8 kDa | |
Human PD-1, His Tag (orb257751) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_005009.2). |
Unconjugated | |
95% | |
48.9 kDa | |
Human PD-L2, Mouse IgG1 Fc Tag (orb334918) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679). |
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