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Catalog Number | orb257767 |
---|---|
Category | Proteins |
Description | Human PD-L2 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679). |
Target | PD-L2 |
Conjugation | Unconjugated |
Tag | C-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAI13679 |
UniProt ID | Q9BQ51 |
MW | 23.4 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.4 kDa. The protein migrates as 34-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Leu 20 - Pro 219 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAI13679 |
SDS-PAGE analysis of Human PD-L2 protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 23.4 kDa after removal of the signal peptide.The apparent molecular mass of PDL2-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
SDS-PAGE: 98.2%; SEC-HPLC: 91.2% | |
24 kDa (predicted); 40-45 kDa (reducing condition, due to glycosylation) |
98.00% | |
25.3 kDa (predicted). Due to glycosylation, the protein migrates to 40-52 kDa based on Tris-Bis PAGE result. |
98.00% | |
25.8 kDa (predicted) |