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Catalog Number | orb392464 |
---|---|
Category | Proteins |
Description | Human SERPINE1 protein |
Target | SERPINE1 |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0 |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0 |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Protein Sequence | VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEPL DHHHHHH |
UniProt ID | P05121 |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Source | Human cells |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Alternative names | Plasminogen Activator Inhibitor-1 protein, Clade E Read more... |
Note | For research use only |
Entrez | 5054 |
Greater than 90% as determined by SDS-PAGE. | |
46.7 kDa | |
E.coli |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.6 kDa after removal of the signal peptide. The apparent molecular mass of PAI1-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE. | |
45.27 kDa |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
42.7 KDa | |
E.Coli |
> 97%, determined by SDS-PAGE | |
This protein contains the human SerpinE1 precursor (NP_0005931) (Met 1-Pro 402) was expressed with a C-terminal polyhistidine tag and expressed from HEK293 Cells. |