Recombinant Human Noggin Protein

SKU: orb3002494

Description

Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.

Key Properties

• Expression System: Human Cells
• Biological Activity: Cell Based Assay: Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP‑4(#orb3002515).（QC verified） Human breast cancer organoids were cultured with Wnt3a(Cat#orb3002512),Noggin (Cat#orb3002494),R-spondin 3 (Cat#orb2993001),KGF(Cat#orb3002467), FGF-10(Cat#orb3002478),EGF (Cat#orb3002493)and NRG1-beta 1(Cat#753). DAPI (blue), Ki67(red) and ER(green). Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#orb3002480) , BMP-4(Cat#orb3002515) , EGF (Cat#orb3002493) , Wnt3a (Cat#orb3002512), Noggin (Cat#orb3002494), R-spondin 1 (Cat#orb3002497) and FGF-4 (Cat#orb3002445) . The organoids showed good morphology.
• Tag: Tag free
• Expression Region: Gln28-Cys232
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• MW: Predicted: 23 KDa. Observed: 25-33 KDa, reducing conditions
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM PB, 500mM NaCl, 2mM EDTA, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Noggin; NOG

Quick Database Links

UniProt

Q13253