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Catalog Number | orb753970 |
---|---|
Category | Proteins |
Description | Recombinant human MMP2 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His Tag |
Purity | Greater than 95% as determined by SDS-PAGE |
MW | 60.6 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
UniProt ID | P08253 |
Source | E.Coli |
Expression System | 109-660 |
Expression Region | 109-660 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 |
Alternative names | 72 kDa gelatinase, 72kD type IV collagenase, CLG 4 Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Greater than 95% as determined by SDS-PAGE. | |
46.8 kDa | |
Mammalian cell |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 71.8 kDa after removal of the signal peptide.The apparent molecular mass of MMP2-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
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