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Human MMP1 protein

SKU: orb392144

Description

Recombinant human MMP1 protein is produced by our Mammalian expression system and the target gene encoding Phe20-Asn469 is expressed with a 6His tag at the C-terminus.

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceFPATLETQEQ DVDLVQKYLE KYYNLKNDGR QVEKRRNSGP VVEKLKQMQE FFGLKVTGKP DAETLKVMKQ PRCGVPDVAQ FVLTEGNPRW EQTHLTYRIE NYTPDLPRAD VDHAIEKAFQ LWSNVTPLTF TKVSEGQADI MISFVRGDHR DNSPFDGPGG NLAHAFQPGP GIGGDAHFDE DERWTNNFRE YNLHRVAAHE LGHSLGLSHS TDIGALMYPS YTFSGDVQLA QDDIDGIQAI YGRSQNPVQP IGPQTPKACD SKLTFDAITT IRGEVMFFKD RFYMRTNPFY PEVELNFISV FWPQLPNGLE AAYEFADRDE VRFFKGNKYW AVQGQNVLHG YPKDIYSSFG FPRTVKHIDA ALSEENTGKT YFFVANKYWR YDEYKRSMDP GYPKMIAHDF PGIGHKVDAV FMKDGFFYFF HGTRQYKFDP KTKRILTLQK ANSWFNCRKN VDHHHHHH
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM MES, 150mM NaCl, 0.05% Brij35, 10% Glycerol, pH 5.5.
DisclaimerFor research use only

Alternative Names

CLG; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human MMP1 protein (orb392144)

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Available Sizes

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10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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