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| Catalog Number | orb257692 |
|---|---|
| Category | Proteins |
| Description | Human MMP-9, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 469 (Accession # AAH06093). It needs to be activated by agents such as APMA in vitro to have hydrolytic activity. |
| Target | MMP-9 |
| Conjugation | Unconjugated |
| Tag | C-6×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 7.5 |
| Purity | 90% |
| Protein Sequence | AAH06093 |
| UniProt ID | P14780 |
| MW | 50.8 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 50.8 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Expression Region | Ala 20 - Pro 469 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH06093 |
SDS-PAGE analysis of Human MMP-9 protein
- Item 1 of 2
Human MMP9(20-707) Protein, His Tag [orb1290924]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 77.2 kDa after removal of the signal peptide. The apparent molecular mass of MMP9(20-707)-His is approximately 70-100 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μg 
Human MMP9(107-707) Protein, His Tag [orb1173830]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 67.4 kDa after removal of the signal peptide. The apparent molecular mass of MMP9-His is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgMMP-9 Protein, Human, Recombinant (His) [orb1958711]
97.70%
77.7 kDa (predicted); 80-95 kDa (reducing condition, due to glycosylation)
20 μg, 100 μg, 200 μg, 500 μg
