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Catalog Number | orb1292386 |
---|---|
Category | Proteins |
Description | Recombinant Human MMP-1 protein |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | C-terminal His Tag |
Purity | Greater than 95% by SDS-PAGE gel analyses |
MW | 50-55 kDa |
Formula | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 |
UniProt ID | P03956 |
Source | HEK 293 cells |
Expression System | 20-469 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4 |
Alternative names | CLG, CLGN, Fibroblast collagenase, Interstitial co Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
69.9 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 75.3 kDa after removal of the signal peptide. The apparent molecular mass of MMP14-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Greater than 85% as determined by SDS-PAGE. | |
24.6 kDa | |
E.coli |
> 97% by SDS-PAGE. | |
KMP1557, Recombinant Human MMP1 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Phe20-Asn469) of human MMP1 (Accession #NP_002412.1) fused with a 6×His Tag at the C-terminus. |
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