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| Catalog Number | orb427102 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human MIF (His C) protein |
| Form/Appearance | Sterile Filtered lyophilized powder. |
| Buffer/Preservatives | Human MIF was lyophilized from a 1mg/ml solution containing PBS pH-7.4. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Protein Sequence | MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFALEHHHHHH |
| Application notes | Cytokines And Growth Factors |
| Source | Escherichia Coli |
| Biological Activity | Measured by its ability to bind rhCD74 in a functional ELISA. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized MIF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized MIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | Phenylpyruvate tautomerase, Glycosylation-inhibiti Read more... |
| Note | For research use only |
>90% as determined by SDS-PAGE | |
55-65 kDa |
>90% as determined by SDS-PAGE. | |
13.45 kDa |
>90% as determined by SDS-PAGE | |
20 kDa |
>90% as determined by SDS-PAGE. | |
13.45 kDa |
Greater than 85.0% as determined by SDS-PAGE. | |
HEK293 |
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