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Catalog Number | orb257689 |
---|---|
Category | Proteins |
Description | Human MICA, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1). |
Target | MICA |
Conjugation | Unconjugated |
Tag | C-7×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAH16929.1 |
UniProt ID | Q29983 |
MW | 33.8 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.8 kDa. The protein migrates as 45-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Glu 24 - Gln 308 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAH16929.1 |
SDS-PAGE analysis of Human MICA protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 12.5 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide. The apparent molecular mass of MICA-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
98.00% | |
34.3 kDa (predicted) |
98.00% | |
25.2 kDa (predicted). Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result. |
98.00% | |
34.4 kDa (predicted). Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result. |