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Human Mesothelin protein
Catalog Number: orb707004
| Catalog Number | orb707004 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Mesothelin Protein is produced by mammalian expression system and the target gene encoding Leu37-Arg286 is expressed with a Fc tag at the C-terminus. |
| Target | MSLN |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Protein Sequence | LAGETGQEAA PLDGVLANPP NISSLSPRQL LGFPCAEVSG LSTERVRELA VALAQKNVKL STEQLRCLAH RLSEPPEDLD ALPLDLLLFL NPDAFSGPQA CTRFFSRITK ANVDLLPRGA PERQRLLPAA LACWGVRGSL LSEADVRALG GLACDLPGRF VAESAEVLLP RLVSCPGPLD QDQQEAARAA LQGGGPPYGP PSTWSVSTMD ALRGLLPVLG QPIIRSIPQG IVAAWRQRSS RDPSWRQPER VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K |
| UniProt ID | Q13421 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Source | Human cells |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | MPF protein, Mesothelin protein, CAK1 antigen prot Read more... |
| Note | For research use only |
| Entrez | 10232 |
- Item 1 of 4
Human Mesothelin (296-580) Protein, mFc-His Tag [orb689392]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 59.3 kDa after removal of the signal peptide.
Mammalian
100 μg, 50 μg, 10 μg - Item 1 of 4
Human Mesothelin(37-286) Protein, His Tag [orb1290953]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of Mesothelin(37-286)-His is approximately 25-35 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 4
Recombinant human Mesothelin, C-hFc (HEK293) [orb1516381]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 68-72 kDa based on Tris-Bis PAGE result.
50 μg - Item 1 of 3
Human Mesothelin protein [orb624114]
Greater than 92% as determined by SDS-PAGE.
59.6 kDa
Mammalian cell
1 mg, 20 μg, 100 μg - Item 1 of 3
Recombinant human Mesothelin, C-His-Avi (HEK293) [orb1516380]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
50 μg
