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Catalog Number | orb707004 |
---|---|
Category | Proteins |
Description | Recombinant Human Mesothelin Protein is produced by mammalian expression system and the target gene encoding Leu37-Arg286 is expressed with a Fc tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | MSLN |
Entrez | 10232 |
UniProt ID | Q13421 |
Protein Sequence | LAGETGQEAA PLDGVLANPP NISSLSPRQL LGFPCAEVSG LSTERVRELA VALAQKNVKL STEQLRCLAH RLSEPPEDLD ALPLDLLLFL NPDAFSGPQA CTRFFSRITK ANVDLLPRGA PERQRLLPAA LACWGVRGSL LSEADVRALG GLACDLPGRF VAESAEVLLP RLVSCPGPLD QDQQEAARAA LQGGGPPYGP PSTWSVSTMD ALRGLLPVLG QPIIRSIPQG IVAAWRQRSS RDPSWRQPER VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | MPF protein, Mesothelin protein, CAK1 antigen prot Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
27.5 kDa | |
Human Mesothelin (37-286), His Tag (orb257696) is expressed from human 293 cells (HEK293). It contains AA Leu 37 - Arg 286 (Accession # AAH09272). |
Greater than 92% as determined by SDS-PAGE. | |
59.6 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
36.7 kDa | |
Mammalian cell |
Greater than 85% as determined by SDS-PAGE. | |
61.2 kDa | |
Mammalian cell |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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