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Catalog Number | orb392131 |
---|---|
Category | Proteins |
Description | Recombinant human MECR protein is produced by our mammalian expression system and the target gene encoding Pro54-Met373 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | MECR |
Entrez | 51102 |
UniProt ID | Q9BV79 |
Protein Sequence | PAKVVELKNL ELAAVRGSDV RVKMLAAPIN PSDINMIQGN YGLLPELPAV GGNEGVAQVV AVGSNVTGLK PGDWVIPANA GLGTWRTEAV FSEEALIQVP SDIPLQSAAT LGVNPCTAYR MLMDFEQLQP GDSVIQNASN SGVGQAVIQI AAALGLRTIN VVRDRPDIQK LSDRLKSLGA EHVITEEELR RPEMKNFFKD MPQPRLALNC VGGKSSTELL RQLARGGTMV TYGGMAKQPV VASVSLLIFK DLKLRGFWLS QWKKDHSPDQ FKELILTLCD LIRRGQLTAP ACSQVPLQDY QSALEASMKP FISSKQILTM VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | NBRF1; Trans-2-enoyl-CoA reductase mitochondrial; Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human MECR(Pro54-Met373) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
35.1 kDa | |
E.Coli |
98.00% | |
39 KDa, reducing conditions |
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