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Catalog Number | orb392128 |
---|---|
Category | Proteins |
Description | Recombinant human MDH1 protein is produced by our E. coli expression system and the target gene encoding Ser2-Ala334 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | MDH1 |
Entrez | 4190 |
UniProt ID | P40925 |
Protein Sequence | SEPIRVLVTG AAGQIAYSLL YSIGNGSVFG KDQPIILVLL DITPMMGVLD GVLMELQDCA LPLLKDVIAT DKEDVAFKDL DVAILVGSMP RREGMERKDL LKANVKIFKS QGAALDKYAK KSVKVIVVGN PANTNCLTAS KSAPSIPKEN FSCLTRLDHN RAKAQIALKL GVTANDVKNV IIWGNHSSTQ YPDVNHAKVK LQGKEVGVYE ALKDDSWLKG EFVTTVQQRG AAVIKARKLS SAMSAAKAIC DHVRDIWFGT PEGEFVSMGV ISDGNSYGVP DDLLYSFPVV IKNKTWKFVE GLPINDFSRE KMDLTAKELT EEKESAFEFL SSALEHHHHH H |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
Alternative names | MDHA; Malate dehydrogenase, cytoplasmic; Cytosolic Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human MDH1(Ser2-Ala334) was fused with the C-terminal His Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
36.6 kDa | |
E.Coli |
98.00% | |
36 KDa, reducing conditions |
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