Human MCAM Protein, His Tag

Catalog Number: orb257287

Catalog Number	orb257287
Category	Proteins
Description	Human MCAM, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 24 - Gly 559 (Accession # NP_006491.2).
Reactivity	Human
Tag	C-6×His
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	60.5 kDa
Target	MCAM
UniProt ID	P43121
Protein Sequence	NP_006491.2
Expression System	HEK293
Biological Origin	Human
Expression Region	Val 24 - Gly 559
Endotoxins	1.0 EU per μg
NCBI	NP_006491.2
Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Buffer/Preservatives	PBS, pH 7.4
Note	For research use only
Application notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.5 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date	6 months from date of receipt.

SDS-PAGE analysis of Human CD146 protein

Purity:	> 97% by SDS-PAGE.
Source:	Recombinant Human CD146/MCAM Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Val24-Gly559) of human CD146/MCAM (Accession #NP_006491.2) fused with a 6��His Tag at the C-terminus.

Sizes Available: 100 μg, 50 μg

Purity:	98.00%
MW:	61.3 kDa (predicted); 88.9 kDa (reducing condition, due to glycosylation)

Purity:	98.00%
MW:	61.3 kDa (predicted); 80-90 kDa (reducing condition, due to glycosylation)

Purity:	> 98%, determined by SDS-PAGE
Source:	This protein contains the extracellular domain of human CD146 precursor (NP_006491.2) (Met 1-Gly 559) was expressed with a C-terminal polyhistidine tag.