Human IgG IgA IgM (H&L) Antibody

SKU: orb347185

Description

Human IgG IgA IgM (H&L) antibody

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:28,800 - 1:48,800, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Human IgG IgA IgM antibody has been tested by ELISA, SDS-PAGE, dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG, IgA and IgM whole molecules
• Purity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat anti Human IgG IgA IgM Antibody, Goat Anti-Human IgG IgA IgM Antibody, Goat Anti-Human IgGAM

ELISA Results of purified Goat Anti-Human IgG, IgA, IgM Antibody tested against purified Human IgG, IgA, IgM. The working dilution for Anti-Human IgG, IgA, IgM is 1:20000. Each well was coated in duplicate with 1.0 µg of Human IgG protein [Red Line], Human IgA [Green Line], Human IgM [Blue Line], Human IgG/A/M [Purple Line]. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated and orb348651 substrate.