Human IgG (H&L) Antibody

SKU: orb347260

Description

Human IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:5,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:500 - 1:5,000
• Reactivity: Human
• Application Notes:
  Mouse Anti-Human IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Purity: This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.98 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mouse Anti-Human IgG Antibody, Mouse Anti Human IgG Antibody

Western Blot of Human IgG Antibody. M: 3 µl Opal Prestained Ladder. Lane 1: Human IgG (p/n orb346219). Load: 50 ng per lane. Primary antibody: Human IgG Antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1:40000 for 30 minutes at RT. Block: (p/n orb348637) for 30 minutes at RT. Predicted/Observed size: 55 kDa, 25 kDa for Human IgG.