Human IgG Fc Antibody

SKU: orb750665

Description

Human IgG F(c)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Human IgG F(c) fragment
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 31.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Human IgG F(c) fragment Antibody, goat anti-Human IgG Fc fragment Antibody, goat anti Human IgG Fc

Western Blot Results of Goat Anti-Human IgG F(c) Antibody. Lane 1: Opal Prestained Molecular Weight. Lane 2: Human F(c) (p/n orb346220) [0.01 µg]. Lane 3: Human IgG whole molecule (p/n orb346219) [0.01 µg]. Lane 4: Human F(ab')2 (orb346221) [0.01 µg]. Primary Antibody: Anti-Human F(c) (GOAT) Antibody at 1:1000 at 2-8°C overnight. Secondary Antibody: Donkey Anti-Goat IgG HRP (p/n orb347032) at 1:40000 for 30 mins at RT. Blocking: BlockOut Blocking buffer (p/n orb348644) for 1hr at RT. Expected MW: ~26kDa F(c).