Human IDH1 protein

Catalog Number: orb706957

Catalog Number	orb706957
Category	Proteins
Description	Recombinant Human IDH1 Protein is produced by E.coli expression system and the target gene encoding Met1-Leu414 is expressed with a 8His tag at the C-terminus.
Tested applications	ELISA, MS, SDS-PAGE, WB
Reactivity	Human
Form/Appearance	Liquid in a 0.2 μm filtered solution of 50mM Tris, 200mM NaCl, 10% glycerol, pH 8.0.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Target	IDH1
Entrez	3417
UniProt ID	O75874
Protein Sequence	MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKLSLEHHH HHHHH
Source	E. coli
Endotoxins	Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Buffer/Preservatives	Liquid in a 0.2 μm filtered solution of 50mM Tris, 200mM NaCl, 10% glycerol, pH 8.0.
Alternative names	PICD protein, Isocitrate dehydrogenase [NADP] cyto Read more...
Note	For research use only
Application notes	Please minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.

Purity:	Greater than 85% as determined by SDS-PAGE.
MW:	47.5 kDa
Source:	E.coli

Applications:	ELISA, MS, SDS-PAGE, WB
Purity:	Greater than 95% as determined by reducing SDS-PAGE.
Source:	E. coli

Purity:	≥90% as determined by SDS-PAGE
Source:	This protein contains the human IDH3(Met1-Leu414(Arg132His)) was fused with the C-terminal His Tag and expressed in E. coli.