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Catalog Number | orb348800 |
---|---|
Category | Proteins |
Description | Human HVEM, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 39 - Val 202 (Accession # Q92956-1). |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 19.2 kDa |
Target | HVEM |
UniProt ID | Q92956 |
Protein Sequence | NP_003811.2 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Leu 39 - Val 202 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_003811.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.2 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human HVEM protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
20.6 kDa (predicted); 39.5 kDa (reducing conditions) |
98.00% | |
19 kDa (predicted) |
98.70% | |
19 kDa (predicted); 33-38 kDa (reducing condition, due to glycosylation) |
> 95% by Tris-Bis PAGE | |
Recombinant Human HVEM Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Pro37-Val202) of Human HVEM fused with hFc His Tag tag at the C-terminal. |