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Catalog Number | orb391832 |
---|---|
Category | Proteins |
Description | Recombinant human HAO1 protein is produced by our E. coli expression system and the target gene encoding Met1-Ile370 is expressed with a Trx, 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | HAO1 |
Entrez | 54363 |
UniProt ID | Q9UJM8 |
Protein Sequence | MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAG SGSGHMHHHH HHSSGLVPRG SGMKETAAAK FERQHMDSPD LGTDDDDKAM ADIGSMLPRL ICINDYEQHA KSVLPKSIYD YYRSGANDEE TLADNIAAFS RWKLYPRMLR NVAETDLSTS VLGQRVSMPI CVGATAMQRM AHVDGELATV RACQSLGTGM MLSSWATSSI EEVAEAGPEA LRWLQLYIYK DREVTKKLVR QAEKMGYKAI FVTVDTPYLG NRLDDVRNRF KLPPQLRMKN FETSTLSFSP EENFGDDSGL AAYVAKAIDP SISWEDIKWL RRLTSLPIVA KGILRGDDAR EAVKHGLNGI LVSNHGARQL DGVPATIDVL PEIVEAVEGK VEVFLDGGVR KGTDVLKALA LGAKAVFVGR PIVWGLAFQG EKGVQDVLEI LKEEFRLAMA LSGCQNVKVI DKTLVRKNPL AVSKI |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | GOX1; HAOX1; Hydroxyacid oxidase 1; HAOX1; Glycola Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human HAO1(Met1-Ile370) was fused with the N-terminal Trx, His Tag and expressed in E. coli. |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
40.6 kDa | |
E.Coli |
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