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Catalog Number | orb706950 |
---|---|
Category | Proteins |
Description | Recombinant Human Glypican 1 Protein is produced by mammalian expression system and the target gene encoding Asp24-Thr529 is expressed fused with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | GPC1 |
Entrez | 2817 |
UniProt ID | P35052 |
Protein Sequence | HHHHHHENLY FQGDPASKSR SCGEVRQIYG AKGFSLSDVP QAEISGEHLR ICPQGYTCCT SEMEENLANR SHAELETALR DSSRVLQAML ATQLRSFDDH FQHLLNDSER TLQATFPGAF GELYTQNARA FRDLYSELRL YYRGANLHLE ETLAEFWARL LERLFKQLHP QLLLPDDYLD CLGKQAEALR PFGEAPRELR LRATRAFVAA RSFVQGLGVA SDVVRKVAQV PLGPECSRAV MKLVYCAHCL GVPGARPCPD YCRNVLKGCL ANQADLDAEW RNLLDSMVLI TDKFWGTSGV ESVIGSVHTW LAEAINALQD NRDTLTAKVI QGCGNPKVNP QGPGPEEKRR RGKLAPRERP PSGTLEKLVS EAKAQLRDVQ DFWISLPGTL CSEKMALSTA SDDRCWNGMA RGRYLPEVMG DGLANQINNP EVEVDITKPD MTIRQQIMQL KIMTNRLRSA YNGNDVDFQD ASDDGSGSGS GDGCLDDLCS RKVSRKSSSS RTPLTHALPG LSEQEGQKT |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | Glypican-1 protein Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 57.0 kDa after removal of the signal peptide. The apparent molecular mass of GPC1-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
90% | |
82.5 kDa | |
Human Glypican 1, Fc Tag (orb612137) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1). |
Unconjugated | |
85% | |
58.0 kDa | |
Human Glypican 1, His Tag (orb612136) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1). |
Unconjugated | |
95% | |
57.9 kDa | |
Human Glypican 1 (S486A, S488A, S490A), His Tag (orb1152430) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # P35052-1 (S486A, S488A, S490A)). |
Unconjugated | |
95% | |
77.9 kDa | |
Human APLP2, His Tag (orb1146983) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 692 (Accession # Q06481-1). |
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