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Catalog Number | orb391812 |
---|---|
Category | Proteins |
Description | Recombinant human GNMT protein is produced by our E. coli expression system and the target gene encoding Met1-Asg294 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | GNMT |
Entrez | 27232 |
UniProt ID | Q14749 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
Alternative names | Glycine N-methyltransferase Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
≥90% as determined by SDS-PAGE | |
This protein contains the human GNMT(Met1-Asg294) was fused with the N-terminal His Tag and expressed in E. coli. |
98% | |
33-37 KDa, reducing conditions |
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