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Catalog Number | orb705305 |
---|---|
Category | Proteins |
Description | Recombinant Human Growth/differentiation factor 7(GDF7) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | > 95 % as determined by SDS-PAGE and HPLC analyses. |
MW | 14.0 kDa |
UniProt ID | Q7Z4P5 |
Protein Sequence | TALAGTRTAQGSGGGAGRGHGRRGRSRCSRKPLHVDFKELGWDDWIIAPLDYEAYHCEGLCDFPLRSHLEPTNHAIIQTLLNSMAPDAAPASCCVPARLSPISILYIDAANNVVYKQYEDMVVEACGCR |
Protein Length | Full Length of Mature Protein |
Source | E.Coli |
Expression System | 322-450aa |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg. |
Expression Region | 322-450aa |
Endotoxins | Less than 0.1 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. |
Alternative names | GDF-7, BMP12 Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
14.1 kDa | |
E.Coli |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
35 KDa | |
E.Coli |
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