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Catalog Number | orb391756 |
---|---|
Category | Proteins |
Description | Recombinant human FN1 protein is produced by our E. coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | FN1 |
Entrez | 2335 |
UniProt ID | P02751 |
Protein Sequence | MPTDLRFTNI GPDTMRVTWA PPPSIDLTNF LVRYSPVKNE EDVAELSISP SDNAVVLTNL LPGTEYVVSV SSVYEQHEST PLRGRQKTGL DSPTGIDFSD ITANSFTVHW IAPRATITGY RIRHHPEHFS GRPREDRVPH SRNSITLTNL TPGTEYVVSI VALNGREESP LLIGQQSTVS DVPRDLEVVA ATPTSLLISW DAPAVTVRYY RITYGETGGN SPVQEFTVPG SKSTATISGL KPGVDYTITV YAVTGRGDSP ASSKPISINY RTEIDKPSMA IPAPTDLKFT QVTPTSLSAQ WTPPNVQLTG YRVRVTPKEK TGPMKEINLA PDSSSVVVSG LMVATKYEVS VYALKDTLTS RPAQGVVTTL ENVSPPRRAR VTDATETTIT ISWRTKTETI TGFQVDAVPA NGQTPIQRTI KPDVRSYTIT GLQPGTDYKI YLYTLNDNAR SSPVVIDAST AIDAPSNLRF LATTPNSLLV SWQPPRARIT GYIIKYEKPG SPPREVVPRP RPGVTEATIT GLEPGTEYTI YVIALKNNQK SEPLIGRKKT DELPQLVTLP HPNLHGPEIL DVPST |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | FN; Fibronectin; FN; Cold-insoluble globulin; CIG Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
23.6 kDa | |
E.coli |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 270.1 kDa after removal of the signal peptide. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
Greater than 95% as determined by SDS-PAGE. | |
62.7 kDa | |
E.coli |
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