Human FEN1 protein

• Catalog Number: orb391718
• Category: Proteins
• Description: Recombinant human FEN1 protein is produced by our E. coli expression system and the target gene encoding Met1-Lys380 is expressed.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 50mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Target: FEN1
• Entrez: 2237
• UniProt ID: P39748
• Protein Sequence: MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
• Source: E. coli
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 50mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0.
• Alternative names: RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap s
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.