Human F10 protein

SKU: orb706934

Description

Recombinant Human Factor X Protein is produced by mammalian expression system and the target gene encoding Asn32-Lys488 is expressed with a Fc tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: F10
• Reactivity: Human
• Protein Sequence: NNILARVTRA NSFLEEMKKG HLERECMEET CSYEEAREVF EDSDKTNEFW NKYKDGDQCE TSPCQNQGKC KDGLGEYTCT CLEGFEGKNC ELFTRKLCSL DNGDCDQFCH EEQNSVVCSC ARGYTLADNG KACIPTGPYP CGKQTLERRK RSVAQATSSS GEAPDSITWK PYDAADLDPT ENPFDLLDFN QTQPERGDNN LTRIVGGQEC KDGECPWQAL LINEENEGFC GGTILSEFYI LTAAHCLYQA KRFKVRVGDR NTEQEEGGEA VHEVEVVIKH NRFTKETYDF DIAVLRLKTP ITFRMNVAPA CLPERDWAES TLMTQKTGIV SGFGRTHEKG RQSTRLKMLE VPYVDRNSCK LSSSFIITQN MFCAGYDTKQ EDACQGDSGG PHVTRFKDTY FVTGIVSWGE GCARKGKYGI YTKVTAFLKW IDRSMKTRGL PKAKSHAPEV ITSSPLKDIE GRMDEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl2, pH 5.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Coagulation factor X; Stuart factor; Stuart-Prower factor

Quick Database Links

Gene Symbol

F10

UniProt

P00742

NCBI Gene ID

2159