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Catalog Number | orb257399 |
---|---|
Category | Proteins |
Description | Human DLL4, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Pro 524 (Accession # Q9NR61-1). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 56.2 kDa |
Target | DLL4 |
UniProt ID | Q9NR61 |
Protein Sequence | AAI06951 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Ser 27 - Pro 524 |
Endotoxins | 0.01 EU per μg |
NCBI | AAI06951 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 56.2 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human DLL4 protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.1 kDa after removal of the signal peptide. The apparent molecular mass of DLL4-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
98.00% | |
57.55 kDa (predicted); 66.88 kDa (reducing conditions) |
98.60% | |
55.7 kDa (predicted); 55-70 kDa (reducing condition, due to glycosylation) |
> 95% by SDS-PAGE. | |
Recombinant Human DLL4 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Ser27-Pro524) of human DLL4 (Accession #Q9NR61) fused with a 10��His Tag at the C-terminus. |
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human DLL4 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Ser27-Pro524) of Human DLL4 fused with a His Tag at the C-terminal. |