Human DKK1 protein

SKU: orb391620

Description

Recombinant human DKK1 protein is produced by our mammalian expression system and the target gene encoding Thr32-His266 is expressed with a 8His tag at the N-terminus.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: DKK1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: HHHHHHHHQT LNSVLNSNAI KNLPPPLGGA AGHPGSAVSA APGILYPGGN KYQTIDNYQP YPCAEDEECG TDEYCASPTR GGDAGVQICL ACRKRRKRCM RHAMCCPGNY CKNGICVSSD QNHFRGEIEE TITESFGNDH STLDGYSRRT TLSSKMYHTK GQEGSVCLRS SDCASGLCCA RHFWSKICKP VLKEGQVCTK HRRKGSHGLE IFQRCYCGEG LSCRIQKDHH QASNSSRLHT CQRH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK

Quick Database Links

Gene Symbol

DKK1

UniProt

O94907

NCBI Gene ID

22943