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Catalog Number | orb594712 |
---|---|
Category | Proteins |
Description | Recombinant Human Granulocyte-macrophage colony-stimulating factor(CSF2) (Active) |
Tag | Tag-Free |
Form/Appearance | Lyophilized powder |
Purity | Greater than 95% as determined by SDS-PAGE. |
MW | 14.6 kDa |
UniProt ID | P04141 |
Protein Sequence | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Expression System | 18-144aa |
Biological Activity | The ED50 as determined in a cell proliferation assay using TF‑1 human erythroleukemic cells is 4-20 pg/ml. |
Expression Region | 18-144aa |
Endotoxins | Less than 0.01 EU/µg as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4. |
Alternative names | Granulocyte-Macrophage Colony-Stimulating Factor; Read more... |
Note | For research use only |
Application notes | Full Length of Mature Protein |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
> 98% as determined by SDS-PAGE and HPLC. | |
14.5 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
15.5 kDa | |
Mammalian cell |
Unconjugated | |
95% | |
14.5 kDa | |
Human GM-CSF, premium grade (orb257510) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - Glu 144 (Accession # NP_000749.2). |
Unconjugated | |
95% | |
20.3 kDa | |
Human IL-37b, His Tag (orb257616) is expressed from E. coli cells. It contains AA Val 46 - Asp 218 (Accession # AAH20637.1). |
Unconjugated | |
95% | |
49.2 kDa | |
Human PD-L2, Fc Tag (HPLC verified) (orb257768) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679). |
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