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Catalog Number | orb750360 |
---|---|
Category | Proteins |
Description | Human Tissue Factor, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Glu 251 (Accession # P13726-1). |
Target | Coagulation Factor III |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 7.5 |
Purity | 90% |
Protein Sequence | NP_001984.1 |
UniProt ID | P13726 |
MW | 26.7 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.7 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Ser 33 - Glu 251 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001984.1 |
> 95% by SDS-PAGE. | |
Recombinant Human Coagulation Factor III/Tissue Factor/CD142 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gly34-Glu251) of human Coagulation Factor III/Tissue Factor/CD142 (Accession #NP_001984.1) fused with a 6��His Tag at the C-terminus. |
> 97%, determined by SDS-PAGE | |
This protein contains the human F3 (P13726) extracellular domain (Met 1-Glu 251) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
Unconjugated | |
90% | |
26.9 kDa |