Human CHIT1 protein

SKU: orb391481

Description

Recombinant human CHIT1 protein is produced by our Mammalian expression system and the target gene encoding Ala22-Asn466 is expressed with a 6His tag at the C-terminus.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: CHIT1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Protein Sequence: AKLVCYFTNW AQYRQGEARF LPKDLDPSLC THLIYAFAGM TNHQLSTTEW NDETLYQEFN GLKKMNPKLK TLLAIGGWNF STQKFTDMVA TANNRQTFVN SAIRFLRKYS FDGLDLDWEY PGSQGSPAVD KERFTTLVQD LANAFQQEAQ TSGKERLLLS AAVPAGQTYV DAGYEVDKIA QNLDFVNLMA YDFHGSWEKV TGHNSPLYKR QEESGAAASL NVDAAVQQWL QKGTPASKLI LGMPTYGRSF TLASSSDTRV GAPATGSGTP GPFTKEGGML AYYEVCSWKG ATKQRIQDQK VPYIFRDNQW VGFDDVESFK TKVSYLKQKG LGGAMVWALD LDDFAGFSCN QGRYPLIQTL RQELSLPYLP SGTPELEVPK PGQPSEPEHG PSPGQDTFCQ GKADGLYPNP RERSSFYSCA GGRLFQQSCP TGLVFSNSCK CCTWNVDHHH HHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chitotriosidase-1; Chitinase-1

Quick Database Links

Gene Symbol

CHIT1

UniProt

Q13231

NCBI Gene ID

1118