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Catalog Number | orb706799 |
---|---|
Category | Proteins |
Description | Recombinant Human Adipsin Protein is produced by mammalian expression system and the target gene encoding Ile26-Ala253 is expressed with a 10His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CFD |
Entrez | 1675 |
UniProt ID | P00746 |
Protein Sequence | ILGGREAEAH ARPYMASVQL NGAHLCGGVL VAEQWVLSAA HCLEDAADGK VQVLLGAHSL SQPEPSKRLY DVLRAVPHPD SQPDTIDHDL LLLQLSEKAT LGPAVRPLPW QRVDRDVAPG TLCDVAGWGI VNHAGRRPDS LQHVLLPVLD RATCNRRTHH DGAITERLMC AESNRRDSCK GDSGGPLVCG GVLEGVVTSG SRVCGNRKKP GIYTRVASYA AWIDSVLAHH HHHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5. |
Alternative names | DF protein, PFD protein, Complement factor D prote Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
26.3 kDa | |
Human Complement Factor D, His Tag (orb612152) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Ala 253 (Accession # P00746-1). |
Unconjugated | |
95% | |
50.8 kDa | |
Human Complement Factor D, Fc Tag (orb612142) is expressed from human 293 cells (HEK293) and inactive. It contains AA Ile 26 - Ala 253 (Accession # P00746-1). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 50.5 kDa after removal of the signal peptide. The apparent molecular mass of CFD-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 95% by SDS-PAGE. | |
KMP1660, Recombinant Human Complement Factor D/Adipsin Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Ile26-Ala253) of human Complement Factor D/Adipsin (Accession #P00746) fused with a 10×His Tag at the C-terminus. |
≥90% as determined by SDS-PAGE | |
This protein contains the human CFD(Ile26-Ala253) was fused with the C-terminal 10- His Tag and expressed in Mammalian cells. |
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