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Human CEACAM5 Protein

Catalog Number: orb1476710

DispatchUsually dispatched within 5-10 working days
$ 180.00
Catalog Numberorb1476710
CategoryProteins
DescriptionRecombinant Human Carcinoembryonic antigen-related cell adhesion molecule 5(CEACAM5)(E398K),partial (Active)
TagC-terminal 10xHis-tagged
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
PurityGreater than 95% as determined by SDS-PAGE.
Protein SequenceKLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSA
Protein LengthFull Length of Mature Protein
UniProt IDP06731
MW74.1 kDa
Application notesWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
EndotoxinsLess than 1.0 EU/ug as determined by LAL method.
SourceMammalian cell
Biological OriginHomo sapiens (Human)
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 at 2μg/mL can bind Anti-CEACAM5 recombinant antibody, the EC50 is 0.8955-1.719 ng/mL.
Expression Region35-685aa(E398K)
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Alternative names(Carcinoembryonic antigen)(CEA)(Meconium antigen 1
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NoteFor research use only
Human CEACAM5 Protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Human CEACAM5 Protein

Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM5 at 2μg/mL can bind Anti-CEACAM5 recombinant antibody, the EC50 is 0.8955-1.719 ng/mL.

  • Recombinant human CEACAM5 protein, C-His (HEK293) [orb1817162]

    > 90% as determined by SDS-PAGE

    72 kDa

    500 μg, 100 μg
  • Anti-CEA [orb2568783]

    ELISA,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    1 mg, 100 μg
  • Anti-CEA [orb2568784]

    ELISA,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    1 mg, 100 μg
  • Anti-CEA [orb2568785]

    ELISA,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    1 mg, 100 μg
  • Human CEACAM5 Protein, His Tag [orb689419]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 72.2 kDa after removal of the signal peptide. The apparent molecular mass of CEACAM5-His is approximately 100-130 kDa due to glycosylation.

    Mammalian

    100 μg, 10 μg, 50 μg