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Catalog Number | orb257269 |
---|---|
Category | Proteins |
Description | Human CD44, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Pro 220 (Accession # AAH04372). |
Reactivity | Human |
Tag | C-9×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 23.3 kDa |
Target | CD44 |
UniProt ID | P16070 |
Protein Sequence | AAH04372 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 21 - Pro 220 |
Endotoxins | 1.0 EU per μg |
NCBI | AAH04372 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.3 kDa. The protein migrates as 32-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD44 protein
Immobilized Human CD44, His Tag at 5 ug/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 39-156 ng/mL (Routinely tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 22.9 kDa after removal of the signal peptide.The apparent molecular mass of CD44-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
98.00% | |
80.2 kDa (predicted) |
95.10% | |
23.4 kDa (predicted); 43-48 kDa (reducing condition, due to glycosylation) |
> 97% by SDS-PAGE. | |
Recombinant Human CD44 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln21-Pro220) of human CD44 (Accession #NP_000601.3) fused with an Fc, 6��His Tag at the C-terminus. |