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Catalog Number | orb762448 |
---|---|
Category | Proteins |
Description | Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1 (CD3E) & AAI13831 (CD3G)). |
Target | CD3E & CD3G |
Conjugation | Unconjugated |
Tag | C-Fc | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_000724.1 (CD3E) & AAI13831 (CD3G) |
MW | 38.3 kDa (CD3E) & 36.9 (CD3G) |
Application notes | Human CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 38.3 kDa (CD3E) and 36.9 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 43-55 kDa under reducing (R) condition, and 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE). |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000724.1, CD3, AAI13831 |
> 90% as determined by SDS-PAGE |