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Human CD31 Protein, His Tag
Catalog Number: orb257255
| Catalog Number | orb257255 |
|---|---|
| Category | Proteins |
| Description | Human CD31, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Lys 601 (Accession # P16284-1). |
| Reactivity | Human |
| Tag | C-10×His |
| Form/Appearance | Powder |
| Purity | 95% |
| Conjugation | Unconjugated |
| MW | 66.4 kDa |
| Target | CD31 |
| UniProt ID | P16284 |
| Protein Sequence | NP_000433.4 |
| Expression System | HEK293 |
| Biological Origin | Human |
| Expression Region | Gln 28 - Lys 601 |
| Endotoxins | 1.0 EU per μg |
| NCBI | NP_000433.4 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 7.5 |
| Note | For research use only |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 66.4 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CD31 protein
CD31/PECAM-1 Protein, Human, Recombinant (aa 28-601, His) [orb1960076]
98.00%
65.6 kDa (predicted). Due to glycosylation, the protein migrates to 80-90 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mgCD31/PECAM-1 Protein, Human, Recombinant (His) [orb1958640]
95.90%
66 kDa (predicted); 100-110 kDa (reducing condition, due to glycosylation)
100 μg, 200 μg, 500 μgRecombinant Human CD31/PECAM1 Protein, His Tag [orb1551390]
> 90% by SDS-PAGE.
Recombinant Human CD31/PECAM1 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gln28-Lys601) of human CD31/PECAM1 (Accession #NP_000433.4) fused with a 6��His Tag at the C-terminus.
100 μg, 50 μg
