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Human CD31 Protein, Fc Tag
Catalog Number: orb257257
| Catalog Number | orb257257 |
|---|---|
| Category | Proteins |
| Description | Human CD31, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Lys 601 (Accession # AAH22512.1). |
| Target | CD31 |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Purity | 95% |
| Protein Sequence | AAH22512.1 |
| UniProt ID | P16284 |
| MW | 90.6 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 90.6 kDa. The protein migrates as 120-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Expression Region | Gln 28 - Lys 601 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | AAH22512.1 |
SDS-PAGE analysis of Human CD31 protein
Human CD31 protein [orb706876]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgHuman PECAM1 Protein, Fc Tag [orb2701844]
> 95%, determined by SDS-PAGE
The mature form of human CD31 (EAW94208.1) extracellular domain (Met1-Lys 601) with five additional amino acids (DDDDK) at the C-terminus was expressed from HEK293 Cells.
50 μg, 10 μgHuman PECAM1 Protein, Fc Tag [orb2701845]
> 97%, determined by SDS-PAGE
This protein contains the extracellular domain (Met 1-Lys 601) of human CD31 (EAW94208.1) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.
200 μg, 100 μgHuman PECAM1 Protein, Fc Tag [orb2701846]
> 95%, determined by SDS-PAGE
This protein contains (Met 1-Lys 601) of human CD31 (EAW942081) was expressed with the fused Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells.
200 μg, 100 μg
