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Human CD274 protein

Catalog Number: orb605286

DispatchUsually dispatched within 1-2 weeks
$ 190.00
Catalog Numberorb605286
CategoryProteins
DescriptionRecombinant Human Programmed cell death 1 ligand 1(CD274),partial
TagC-terminal hFc-tagged
Form/AppearanceLyophilized powder
Buffer/PreservativesLyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
PurityGreater than 95% as determined by SDS-PAGE.
Protein SequenceFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER
Protein LengthPartial
UniProt IDQ9NZQ7
MW52.7 kDa
Application notesExtracellular Domain
EndotoxinsLess than 1.0 EU/ug as determined by LAL method.
SourceMammalian cell
Biological OriginHomo sapiens (Human)
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody, the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.
Expression Region19-238aa
StorageThe shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Alternative namesPD-L1 (PDCD1 ligand 1) (Programmed death ligand 1)
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NoteFor research use only
Human CD274 protein

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Human CD274 protein

Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody, the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.

Human CD274 protein

The purity of CD274 was greater than 90% as determined by SEC-HPLC.

  • Human PD-L1 Protein, mFc-His tag [orb689384]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.

    Mammalian

    100 μg, 10 μg, 50 μg
  • Recombinant human PD-L1 protein, C-His (HEK293) [orb1516352]

    > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC

    Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.

    100 μg
  • Recombinant human PD-L1 protein, C-mFc (HEK293) [orb1516351]

    > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC

    Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result.

    100 μg
  • Human PD-L1 Protein, His tag [orb689464]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 26.0 kDa after removal of the signal peptide.The apparent molecular mass of PDL1-His is approximately 33-35 kDa due to glycosylation.

    Mammalian

    10 μg, 100 μg, 50 μg
  • Human PD-L1(19-132) Protein, hFc Tag [orb2321289]

    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 39.2 kDa after removal of the signal peptide. The apparent molecular mass of PD-L1(19-132)-hFc is approximately 35-55 kDa due to glycosylation.

    Mammalian

    10 μg, 50 μg, 100 μg