Human CD274 protein

• Catalog Number: orb391393
• Category: Proteins
• Description: Recombinant human CD274 protein is produced by our mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a 6His tag at the C-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Target: CD274
• Entrez: 29126
• UniProt ID: Q9NZQ7
• Protein Sequence: FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER TVDHHHHHH
• Source: Human cells
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Buffer/Preservatives: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, pH 7.4.
• Alternative names: B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell dea
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.

Immobilized Anti-CD274 antibody at 2 μg/ml (100 μl/well) can bind Recombinant Human CD274 Protein. The ED50 of Recombinant Human CD274 Protein is 0.0094 μg/ml.