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Catalog Number | orb754317 |
---|---|
Category | Proteins |
Description | Recombinant human Carbonic Anhydrase 4 protein |
Tag | N-terminal His Tag or N-terminal His-IF2DI Tag, determined during production process |
Reactivity | Human |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
Purity | Greater than 95% as determined by SDS-PAGE |
UniProt ID | P22748 |
MW | 29 kDa |
Tested applications | ELISA, WB |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Source | E.Coli |
Expression Region | 19-283 |
Storage | -20°C for 12 months as lyophilized;2-8°C for 1 month under sterile conditions after reconstitution |
Alternative names | CA IV, CA4, CAH4_HUMAN, CAIV, Car4, Carbonate dehy Read more... |
Research Area | Epigenetics |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human CA4(Ala19-Lys283) was fused with the C-terminal His Tag and expressed in E. coli. |
31.7 kDa (predicted); 34 and 19 kDa (reducing conditions) |
>95%, determined by SDS-PAGE | |
This protein contains the human CA4 (NP_0007081) (Met1-Lys283) without the pro peptide was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |