You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb257233 |
---|---|
Category | Proteins |
Description | Human CADM1, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 45 - His 374 (Accession # NP_055148.3). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 37.9 kDa |
Target | CADM1 |
UniProt ID | Q9BY67 |
Protein Sequence | NP_055148.3 |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 45 - His 374 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_055148.3 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | PBS, pH 7.4 |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.9 kDa. The protein migrates as 50 kDa and 55-85 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human CADM1 protein
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CADM1-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
97.50% | |
38.5 kDa (predicted); 70-80 kDa (reducing condition, due to glycosylation) |
> 97% by SDS-PAGE. | |
Recombinant human CADM1/IGSF4A Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Gln45-His Tag374) of human CADM1/IGSF4A (Accession #NP_055148.3) fused with a 6��His Tag at the C-terminus. |
> 94%, determined by SDS-PAGE | |
This protein contains the human CADM1 (NP_0551483) extracellular domain (Met 1-His 374) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
> 96%, determined by SDS-PAGE | |
This protein contains the human CADM1 (NP_0551483) extracellular domain (Met 1-His 374) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |